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VOL. 1, ISSUE 1 (2025)
Isolation and identification of cyanobacterial species from selected Regions in India
Authors
Rahul Gaykhe
Abstract
Cyanobacteria are pivotal
primary producers and vital components of aquatic and terrestrial ecosystems,
yet their diversity in many regions of India remains underexplored. This study
aimed to isolate, purify, and identify dominant cyanobacterial species from
diverse ecological niches—including freshwater lakes, rice paddy fields, and
coastal regions of Kerala, Uttar Pradesh, and Odisha. Samples were collected
aseptically and enriched in nitrogen-free BG-11 medium under controlled
laboratory conditions. Axenic cultures were obtained using streak plate and
serial dilution methods, coupled with treatment with antibiotics (cycloheximide
and penicillin). Morphological identification was performed using light
microscopy based on characteristics such as trichome morphology, cell
dimensions, presence of heterocysts and akinetes, and branching patterns. A
total of 15 cyanobacterial isolates were successfully purified and identified
to the generic level. The isolates belonged to the orders Nostocales and
Oscillatoriales, with dominant genera being Oscillatoria, Nostoc, Anabaena, Phormidium,
and Westiellopsis. The prevalence of heterocystous genera in agricultural
soils suggests a significant role in biological nitrogen fixation. This culture
collection provides a valuable resource for further biotechnological
applications in agriculture, biofuel production, and pharmaceutical research.
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Pages:17-22
How to cite this article:
Rahul Gaykhe "Isolation and identification of cyanobacterial species from selected Regions in India". World Journal of Botany, Vol 1, Issue 1, 2025, Pages 17-22
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