Background: Soil salinization is among the most pervasive
abiotic constraints on crop productivity in arid and semi-arid agricultural
zones, with mungbean (Vigna radiata L.) being particularly sensitive
among grain legumes. Silicon (Si) has been reported to alleviate salt-induced
physiological damage in several monocot and dicot species, although its role in
mungbean remains comparatively under-studied.
Objective: This study evaluated the influence of graded
NaCl-induced salinity (0, 50, 100, and 150 mM) and exogenous silicon
supplementation (100 mM NaCl + Si) on shoot elongation, total chlorophyll
content, and biomass partitioning in mungbean seedlings under controlled
conditions.
Method: A completely randomized design (CRD) with five
treatments and fifteen replicates per treatment (n = 75) was used. This study
uses a simulated dataset created for academic training purposes; values were
generated to reflect physiologically plausible response patterns documented in
the salinity-tolerance literature and do not represent observations from an
actual laboratory trial. Shoot length was measured at day 21, total chlorophyll
content was assessed spectrophotometrically at five time points, and biomass
allocation among root, shoot, leaf, and reproductive tissue was recorded
destructively at harvest.
Key Results: Shoot length declined progressively with increasing
NaCl concentration, from 18.4 cm in controls to 6.9 cm at 150 mM NaCl, while
co-application of silicon with 100 mM NaCl partially restored growth to 13.7
cm. Total chlorophyll content under 100 mM NaCl fell from 2.08 to 0.98 mg g⁻¹
FW over 21 days, whereas silicon-treated plants retained 1.55 mg g⁻¹ FW at the
same concentration. One-way ANOVA indicated significant treatment effects on
shoot length (p < 0.001).
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