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VOL. 2, ISSUE 1 (2026)
Effects of salinity stress and exogenous silicon amendment on growth, chlorophyll content, and biomass allocation in Vigna radiata seedlings
Authors
Dr. Priya Sharma, Dr. Miguel Alvarez
Abstract

Background: Soil salinization is among the most pervasive abiotic constraints on crop productivity in arid and semi-arid agricultural zones, with mungbean (Vigna radiata L.) being particularly sensitive among grain legumes. Silicon (Si) has been reported to alleviate salt-induced physiological damage in several monocot and dicot species, although its role in mungbean remains comparatively under-studied.

Objective: This study evaluated the influence of graded NaCl-induced salinity (0, 50, 100, and 150 mM) and exogenous silicon supplementation (100 mM NaCl + Si) on shoot elongation, total chlorophyll content, and biomass partitioning in mungbean seedlings under controlled conditions.

Method: A completely randomized design (CRD) with five treatments and fifteen replicates per treatment (n = 75) was used. This study uses a simulated dataset created for academic training purposes; values were generated to reflect physiologically plausible response patterns documented in the salinity-tolerance literature and do not represent observations from an actual laboratory trial. Shoot length was measured at day 21, total chlorophyll content was assessed spectrophotometrically at five time points, and biomass allocation among root, shoot, leaf, and reproductive tissue was recorded destructively at harvest.

Key Results: Shoot length declined progressively with increasing NaCl concentration, from 18.4 cm in controls to 6.9 cm at 150 mM NaCl, while co-application of silicon with 100 mM NaCl partially restored growth to 13.7 cm. Total chlorophyll content under 100 mM NaCl fell from 2.08 to 0.98 mg g⁻¹ FW over 21 days, whereas silicon-treated plants retained 1.55 mg g⁻¹ FW at the same concentration. One-way ANOVA indicated significant treatment effects on shoot length (p < 0.001).

Conclusion: Silicon supplementation mitigated, but did not fully reverse, salinity-induced suppression of vegetative growth and photosynthetic pigment content in mungbean, supporting its candidacy as a low-cost amendment for salt-affected cropping systems pending validation with field-derived data.
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Pages:1-5
How to cite this article:
Dr. Priya Sharma, Dr. Miguel Alvarez "Effects of salinity stress and exogenous silicon amendment on growth, chlorophyll content, and biomass allocation in <i>Vigna radiata</i> seedlings". World Journal of Botany, Vol 2, Issue 1, 2026, Pages 1-5
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